Recombinant Human MANF 0 ReviewsSubmit a Review Product Details Catalog Number: 450-06 Description: MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote the survival, growth and function of dopamine-specific neurons. MANF and its structural homolog CDNF each contain a N-terminal, saposin-like, lipid-binding domain, and a carboxyl-terminal domain that is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA-induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson's disease. Recombinant Human MANF is an 18.1 kDa protein consisting of 158 amino acids, including 8 cysteine residues. Source: E.coli Synonyms: Mesencephalic Astrocyte-derived Neurotrophic Factor, ARMET, Arginine-rich protein (ARP) AA Sequence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 μg/ml. Calculated Molecular Weight: 18.1 kDa Accession Number: P55145 Gene ID: 7873 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Neurobiology Stem Cells & Differentiation Recent Citations First Author Wu, T Title Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway. Citation The Journal of Experimental Medicine; 218(6) PubMed ID 33856409 First Author Gao, F Title Identification of Mesencephalic Astrocyte-Derived Neurotrophic Factor as a Novel Neuroprotective Factor for Retinal Ganglion Cells. Citation Frontiers in Molecular Neuroscience; 10 pg76 PubMed ID 28367115
