Recombinant Human R-Spondin-2 0 ReviewsSubmit a Review Product Details Catalog Number: 120-43 Description: The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally-related, secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. This activity effectively activates and increases the subsequent nuclear signaling of β-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/β-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant Human R-Spondin-2 is a 24.4 kDa protein consisting of 212 amino acid residues. Due to glycosylation, R-Spondin-2 migrates at an apparent molecular weight of approximately 30.0 kDa by SDS PAGE analysis under reducing conditions. Source: CHO cells Synonyms: Roof plate-specific spondin-2, RSPO2 AA Sequence: ASYVSNPICK GCLSCSKDNG CSRCQQKLFF FLRREGMRQY GECLHSCPSG YYGHRAPDMN RCARCRIENC DSCFSKDFCT KCKVGFYLHR GRCFDECPDG FAPLEETMEC VEGCEVGHWS EWGTCSRNNR TCGFKWGLET RTRQIVKKPV KDTILCPTIA ESRRCKMTMR HCPGGKRTPK AKEKRNKKKK RKLIERAQEQ HSVFLATDRA NQ Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Biological Activity: R-Spondin-2 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED50 for this effect is 0.8 – 2.0 μg/ml. Calculated Molecular Weight: 24.4 kDa Accession Number: Q6UXX9 Gene ID: 340419 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Human References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Lipid Metabolism Neurobiology Stem Cells & Differentiation Recent Citations First Author Lee, H Title R-spondins are BMP receptor antagonists in Xenopus early embryonic development Citation Nature Communications; 11(1) pg5570 PubMed ID 33149137 First Author Chang, T Title LGR5+ epithelial tumor stem-like cells generate a 3D-organoid model for ameloblastoma. Citation Cell death & disease; 11(5) pg338 PubMed ID 32382005