Recombinant Murine EGF

Product Details

Catalog Number: 315-09

Description:

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant Murine EGF is a 6.0 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds.

Source: E.coli

Synonyms: Epidermal Growth Factor, Urogastrone, URG

AA Sequence: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED₅₀ was determined by a cell proliferation assay using BALB/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

Calculated Molecular Weight: 6 kDa

Accession Number: P01132

Gene ID: 13645

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

Cross Reactivity Cited in References:

Bacteria, Dog, Guinea Pig, Human, Mouse, Pig, Rat

Country Of Origin: USA

Not for human use.

Research Interest

Organoids

Angiogenesis/Cardiovascular

Stem Cells & Differentiation

Wound Healing

Diabetes/Weight Regulation

Recent Citations

First Author

Sakuma, R

Title

Brain Pericytes Acquire Stemness via the Nrf2-Dependent Antioxidant System.

Citation

Stem cells (Dayton, Ohio); 40(7) pg641-654

PubMed ID

35353891

First Author

Tsubakihara, Y

Title

TGFβ selects for pro-stemness over pro-invasive phenotypes during cancer cell epithelial-mesenchymal transition.

Citation

Molecular Oncology; 16(12) pg2330-2354

PubMed ID

35348275

First Author

Cambuli, F

Title

Intra-epithelial non-canonical Activin A signaling safeguards prostate progenitor quiescence.

Citation

EMBO reports; 23(5) pge54049

PubMed ID

35253958

Product Reviews

Existing reviews

EGF

Works well for cell culture

From: Yu | Date: 7/11/2020

Helps grow patient derived stem cells

Extremely helpful in keeping the stem cell characteristics of patient-derived stem cells unchanged.

From: Shrita | Date: 8/26/2020

Organoid Media supplement growth factor

We have been using this product in our lab as a supplement in the media for growing organoids derived from a mouse prostate. It is a really helpful product and helps for the 3D organoids to form isolated from the epithelial tissue of the mouse prostate. The delivery of the product is also very quick and that's why we prefer buying it from peprotech.

From: Tejasveeta | Date: 8/27/2020

Quality product - no other match!

We routinely use recombinant EGF for maintaining our brain cancer cells. There is no match for the quality that Peprotech provides. Even after their move to a different location or during this pandemic the supply did not interrupt. That helped us to keep our research going! Keep it up!

From: Munjal | Date: 8/28/2020

Growth Factors & Cytokines