Recombinant Murine GDNF 0 ReviewsSubmit a Review Product Details Catalog Number: 450-44 Description: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional murine GDNF ligand is a disulfide-linked homodimer consisting of two 15.1 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Murine GDNF is 30.2 kDa. Source: E.coli Synonyms: Glial-Derived Neurotrophic Factor, ATF-1 AA Sequence (monomer): MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by the proliferation of rat C6 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg. Calculated Molecular Weight: 30.2 kDa Accession Number: P48540 Gene ID: 14573 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Human, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Cell Culture Immune System Neurobiology Stem Cells & Differentiation TGF-β Superfamily Recent Citations First Author Walter, J Title The Parkinson's-disease-associated mutation LRRK2-G2019S alters dopaminergic differentiation dynamics via NR2F1. Citation Cell Reports; 37(3) pg109864 PubMed ID 34686322 First Author Rore, H Title Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells Citation Communications Biology; 4(1) pg802 PubMed ID 34183774 First Author Yuan, H Title Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Citation Stem Cells Translational Medicine; 10(6) pg922-938 PubMed ID 33481357
