Recombinant Murine SCF 2 ReviewsSubmit a Review Product Details Catalog Number: 250-03 Description: SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Murine SCF is an 18.3 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Source: E.coli Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor AA Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg. Calculated Molecular Weight: 18.3 kDa Accession Number: P20826 Gene ID: 17311 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Human, Human + Hamster, Monkey, Mouse, Mouse + Parasite, Mouse + Virus, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Stem Cells & Differentiation Recent Citations First Author Dai, Y Title Meteorin links the bone marrow hypoxic state to hematopoietic stem/progenitor cell mobilization. Citation Cell Reports; 40(12) pg111361 PubMed ID 36130501 First Author Nayak, R C Title Nuclear Vav3 is required for polycomb repression complex-1 activity in B-cell lymphoblastic leukemogenesis. Citation Nature Communications; 13(1) pg3056 PubMed ID 35650206 First Author Teegala, L R Title Protein Kinase C α and β compensate for each other to promote stem cell factor-mediated KIT phosphorylation, mast cell viability and proliferation. Citation FASEB journal : official publication of the Federation of American Societies for Experimental Biology; 36(5) pge22273 PubMed ID 35349200 Product Reviews Existing reviews Recombinant Murine SCF We used this when we ran out of L929-conditioned medium to produce bone marrow derived macrophages. Works equally well. Good to have as a backup. From: MCBL | Date: 8/7/2020 × Recombinant Murine SCF We used this when we ran out of L929-conditioned medium to produce bone marrow derived macrophages. Works equally well. Good to have as a backup. From: MCBL | Date: 8/7/2020 Close Recombinant Murine SCF We use this product to in our media. Works well From: Nichole | Date: 8/26/2020 × Recombinant Murine SCF We use this product to in our media. Works well From: Nichole | Date: 8/26/2020 Close
