Biotinylated Anti-Human sFas Ligand 0 BewertungenSubmit a Review Produktdetails Katalognummer: 500-P184GBT Beschreibung: Source: Polyclonal Goat Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL/Apo1L. Anti-Human FasL/Apo1L specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: CHO cells derived Recombinant Human sFas Ligand (PeproTech catalog# 310-03)* *Catalog# 310-03 has been replaced with 310-03H. Sandwich ELISA: To detect hFasL/Apo1L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human FasL/Apo1L (500-P184G) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hFasL/Apo1L. Western Blot: To detect hFasL/Apo1L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL/Apo1L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. SDS Datenblattsuche Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Immune System TNF Superfamily
