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Biotinylierte Polyklonale Antikörper

Biotinylated Anti-Murine IL-10

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Produktdetails

Katalognummer: 500-P60BT
Beschreibung:
Biotinylated Anti-Murine IL-10
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-10. Anti-Murine IL-10 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Murine IL-10 (PeproTech catalog# 210-10)

Sandwich ELISA: To detect mIL-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine IL-10 (500-P60) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-10.

Biotinylated Anti-Murine IL-10 Sandwich ELISA

Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Murine IL-10 Western Blot Reduced Biotinylated Anti-Murine IL-10 Western Blot Unreduced

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

Erstautor
Verginis, P
Titel
Delineation of five thyroglobulin T cell epitopes with pathogenic potential in experimental autoimmune thyroiditis.
Literaturstelle
Journal of immunology (Baltimore, Md. : 1950); 169(9) pg5332-7
PubMed ID