Animal-Free Recombinant Human IGF-I 0 AvisSubmit a Review Détails du Produit Catalog Number: AF-100-11 Description: The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant Human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. The calculated molecular weight of Recombinant Human IGF-I is 7.6 kDa. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Insulin-like Growth Factor-I, Somatamedin C, IGF-IA AA Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 7.6 kDa Accession Number: P05019 Gene ID: 3479 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Bacteria, Chicken, Cow, Frog, Horse, Human, Monkey, Mouse, Pig, Rat, Salamander, Sheep References PubMed SDS Recherche de CoA Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Bone, Skeletal, Cartilage Diabetes/Weight Regulation Immune System Inflammation Neurobiology Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations Premier Auteur Sissaoui, S Titre Hyperglycaemia up-regulates placental growth factor (PlGF) expression and secretion in endothelial cells via suppression of PI3 kinase-Akt signalling and activation of FOXO1 Citation Scientific Reports; 11(1) pg16344 Id PubMed 34381074 Premier Auteur James, O G Titre iPSC-derived myelinoids to study myelin biology of humans. Citation Developmental Cell; 56(9) pg1346-1358.e6 Id PubMed 33945785 Premier Auteur García-León, J A Titre Generation of oligodendrocytes and establishment of an all-human myelinating platform from human pluripotent stem cells. Citation Nature Protocols; 15(11) pg3716-3744 Id PubMed 33097924