Recombinant Human sRANK Ligand (E.coli derived) 0 AvisSubmit a Review Détails du Produit Catalog Number: 310-01 Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK+ T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Human RANKL is reactive on murine cells. Recombinant Human sRANKL is a 20.0 kDa polypeptide comprising the TNF-homologous region of RANKL (176 amino acid residues). Source: E.coli Synonyms: soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor) AA Sequence: MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells. Calculated Molecular Weight: 20 kDa Accession Number: O14788 Gene ID: 8600 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Human + Cow, Mouse, Rat References PubMed SDS Recherche de CoA Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Bone, Skeletal, Cartilage Cancer Diabetes/Weight Regulation Immune System TNF Superfamily product.subtitle.recentcitations Premier Auteur Borciani, G Titre Protocol of Co-Culture of Human Osteoblasts and Osteoclasts to Test Biomaterials for Bone Tissue Engineering. Citation Methods And Protocols; 5(1) Id PubMed 35076543 Premier Auteur Kaifu, T Titre DCIR and its ligand asialo-biantennary N-glycan regulate DC function and osteoclastogenesis. Citation The Journal of Experimental Medicine; 218(12) Id PubMed 34817551 Premier Auteur Zhang, J Titre In vitro and in vivo detection of tunneling nanotubes in normal and pathological osteoclastogenesis involving osteoclast fusion Citation Laboratory investigation; a journal of technical methods and pathology; 101(12) pg1571-1584 Id PubMed 34537825