Animal-Free Recombinant Murine G-CSF

Détails du Produit

Catalog Number: AF-250-05

Description:

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF is cross-species reactive. Recombinant Murine G-CSF is a 19.0 kDa protein consisting of 179 amino acid residues.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Granulocyte Colony-Stimulating, Factor, CSF-3, MGI-1G, GM-CSFβ, pluripoietin

AA Sequence: MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to stimulate the proliferation of mouse NFS-60 cells. The expected ED₅₀ is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.

Calculated Molecular Weight: 19 kDa

Accession Number: P09920

Gene ID: 12985

Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug)

crossreactivity:

Mouse, Rat