Recombinant Murine Leptin

Détails du Produit

Catalog Number: 450-31

Description:

Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Murine Leptin is a 16.2 kDa protein containing 147 amino acid residues.

Source: E.coli

Synonyms: Obesity protein (OB)

AA Sequence: MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: PeproTech's Murine Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

Calculated Molecular Weight: 16.2 kDa

Accession Number: P41160

Gene ID: 16846

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

crossreactivity:

Hamster, Human, Monkey, Mouse, Pig, Rat