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抗原亲和纯化的多克隆抗体

Anti-Human Nanog

产品详情

产品编号: 500-P236
产品描述:
Anti-Human Nanog
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNanog. Anti-Human Nanog specific antibody was purified by affinity chromatography employing immobilized hNanog matrix.

Immunogen: E.coli derived Recombinant Human Nanog (PeproTech catalog# 120-21)

Sandwich ELISA: To detect hNanog by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Nanog (500-P236Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog.

Anti-Human Nanog Sandwich ELISA

Western Blot: To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human Nanog Western Blot Reduced Anti-Human Nanog Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

第一作者
Xu, W
标题
Methanol fixed feeder layers altered the pluripotency and metabolism of bovine pluripotent stem cells.
文献引用
Scientific Reports; 12(1) pg9177
PubMed Id
第一作者
Li, N
标题
p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation.
文献引用
Stem Cells International; 2021 pg4968649
PubMed Id
第一作者
De Clerck, L
标题
Untargeted histone profiling during naive conversion uncovers conserved modification markers between mouse and human.
文献引用
Scientific Reports; 9(1) pg17240
PubMed Id

product.subtitle.productreviews

现有评论
bovine embryos and derived embryonic stem cells
This product works really good in in vitro bovine embryos and embryonic stem cells derived on MEFs. Perfect to validate pluripotency
来自:  Nicolás | 日期:  2020/7/9