生物素标记的多克隆抗体

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human TFF-2.  Anti-Human TFF-2 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli-derived, 12.0 kDa Recombinant Human TFF-2 (PeproTech catalog# 300-59)

Sandwich ELISA: To detect Human TFF-2 by sandwich ELISA (using 100 μl/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti- Human TFF-2 (500-P312) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human TFF-2.

Western Blot: To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human TFF-2 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.