Recombinant Human BMP-4 (HeLa derived)

产品详情

产品编号： 120-05

产品描述：

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. Members of this superfamily are widely distributed throughout the body, and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 is involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Recombinant Human BMP-4, expressed in HeLa cells, is a 25.6 kDa homodimeric glycoprotein.

Source: HeLa cells

Synonyms: Bone Morphogenetic Protein-4, BMP-2B, DVR4

AA Sequence (monomer): HHSQRARKKN KNCRRHSLYV DFSDVGWNDW IVAPPGYQAF YCHGDCPFPL ADHLNSTNHA IVQTLVNSVN SSIPKACCVP TELSAISMLY LDEYDKVVLK NYQEMVVEGC GCR

Purity: ≥ 97% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED₅₀ for this effect is 2-8 ng/ml.

Calculated Molecular Weight: 25.6 kDa

Accession Number: P12644

Gene ID: 652

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

Note:

100μg is the largest vial size for this product.
Larger sizes supplied as multiple 50μg and/or 100μg vials.

crossreactivity:

Human, Mouse, Mouse + Rat, Pig, Rat

Country Of Origin: USA

Not for human use.

Research Interest

Organoids

血管生成/心血管

细胞凋亡

骨/骨骼系统/软骨

细胞培养

免疫系统

神经生物学

干细胞&分化

TGF-β超家族

product.subtitle.recentcitations

第一作者

Kitajima, K

标题

An Interferon-γ/FLT3 Axis Positively Regulates Hematopoietic Progenitor Cell Expansion from Human Pluripotent Stem Cells.

文献引用

Stem cells (Dayton, Ohio); 40(10) pg906-918

PubMed Id

35901509

第一作者

van der Vaart, J

标题

Modelling of primary ciliary dyskinesia using patient-derived airway organoids.

文献引用

EMBO reports; 22(12) pge52058

PubMed Id

34693619

第一作者

Pouyanfard, S

标题

Human induced pluripotent stem cell-derived macrophages ameliorate liver fibrosis.

文献引用

Stem cells (Dayton, Ohio); 39(12) pg1701-1717

PubMed Id

34460131

product.subtitle.productreviews

现有评论

High-yield iPSC differentiation!

When differentiating stem cells, I always turn to Peprotech for ordering my growth factors (like BMP4). They stay stable for long periods of time under the recommended storage conditions. And they hold up well in the differentiation media, because I get good differentiation results without excessive cell death. Results are consistent between differentiations, so I appreciate this reproducibility. ... reviews.showmore

来自: Kendall | 日期: 2020/8/26

iPSC-Microglia differentiation

When I differentiate iPSC into microglia, I always use peprotech products. It is important to have a good quality of cytokines and growth factors to yield microglia else the iPSC would undergo spontaneous differentiation and non-myeloidal lineages. With BMP-4 from peprotech, I get good consistency in generating mesodermal cells and high yield microglial progenitors cells in large quantities.

来自: Raghavendra | 日期: 2021/8/24

生长因子和细胞因子