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生长因子和细胞因子

Animal-Free Recombinant Human EGF

产品详情

产品编号: AF-100-15
产品描述:
Animal-Free Recombinant Human EGF

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant Human EGF is a 6.2 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds.

*Animal-Free Recombinant Human EGF (Catalog Number AF-100-15) has replaced Recombinant Human EGF (Catalog Number 100-15)

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Epidermal Growth Factor, Urogastrone, URG

AA Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Recombinant Human EGF Biological Activity Graph Recombinant Human EGF WHO Comparison Graph

Calculated Molecular Weight: 6.2 kDa

Accession Number: P01133

Gene ID: 1950

Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug)

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

第一作者
Niu, Y
标题
Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select.
文献引用
Nature Genetics; 54(12) pg1983-1993
PubMed Id
第一作者
Hanson, R L
标题
Coordination of MAPK and p53 dynamics in the cellular responses to DNA damage and oxidative stress.
文献引用
Molecular Systems Biology; 18(12) pge11401
PubMed Id
第一作者
Zhang, Z
标题
Ti3 C2 Tx MXene Composite 3D Hydrogel Potentiates mTOR Signaling to Promote the Generation of Functional Hair Cells in Cochlea Organoids.
文献引用
Advanced science (Weinheim, Baden-Wurttemberg, Germany); 9(32) pge2203557
PubMed Id

product.subtitle.productreviews

现有评论
Quality EGF for Organoid Culture
one of best quality growth factors from peprotech, we are using for various organoid media. Consistent quality, very very cost effective than competitor suppliers, next day delivery.
来自:  Hardik | 日期:  2020/8/14
Constant quality for in vitro culture
Using for in vitro culture. Constant quality. Also quick delivery.
来自:  KOHSUKE | 日期:  2021/8/24
EGF for primary cell culture
I have used Peprotech human recombinant EGF and FGF for the culture of primary glioblastoma cells for several years now. Works well, no complaints and any questions I have had have been answered quickly and thoroughly by Peprotech customer service
来自:  Kathleen | 日期:  2021/8/24
EGF, human basic recombinant, E. coli
Using the EGF for 5 years now in our culture medium and are very satisfied ! Critical cells, but they grow very well with the EGF (and FGF) of Peprotech !
来自:  Arjan | 日期:  2021/8/27
Great recombinant protein
Works very nicely in combination with FGF to keep mouse ESC-derived NPCs alive and proliferating in culture (at 10 ng/ml concentration). Highly recommend.
来自:  Epp | 日期:  2021/8/31