Recombinant Human sRANK Ligand (E.coli derived) 0 评论Submit a Review 产品详情 产品编号: 310-01 产品描述: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK+ T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Human RANKL is reactive on murine cells. Recombinant Human sRANKL is a 20.0 kDa polypeptide comprising the TNF-homologous region of RANKL (176 amino acid residues). Source: E.coli Synonyms: soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor) AA Sequence: MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells. Calculated Molecular Weight: 20 kDa Accession Number: O14788 Gene ID: 8600 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Human + Cow, Mouse, Rat References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest 细胞凋亡 骨/骨骼系统/软骨 癌症 糖尿病/体重调节 免疫系统 TNF超家族 product.subtitle.recentcitations 第一作者 Borciani, G 标题 Protocol of Co-Culture of Human Osteoblasts and Osteoclasts to Test Biomaterials for Bone Tissue Engineering. 文献引用 Methods And Protocols; 5(1) PubMed Id 35076543 第一作者 Kaifu, T 标题 DCIR and its ligand asialo-biantennary N-glycan regulate DC function and osteoclastogenesis. 文献引用 The Journal of Experimental Medicine; 218(12) PubMed Id 34817551 第一作者 Zhang, J 标题 In vitro and in vivo detection of tunneling nanotubes in normal and pathological osteoclastogenesis involving osteoclast fusion 文献引用 Laboratory investigation; a journal of technical methods and pathology; 101(12) pg1571-1584 PubMed Id 34537825