Animal-Free Recombinant Human TNF-α 0 评论Submit a Review 产品详情 产品编号: AF-300-01A 产品描述: TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolytically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin AA Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. Calculated Molecular Weight: 17.4 kDa Accession Number: P01375 Gene ID: 7124 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Cow, Dog, Human, Human + Virus, Monkey, Mouse, Pig, Rat, Sheep References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 艾滋病/HIV 血管生成/心血管 细胞凋亡 癌症 糖尿病/体重调节 免疫系统 炎症 神经生物学 干细胞&分化 TNF超家族 过敏 移植 product.subtitle.recentcitations 第一作者 Laucirica, D R 标题 Pseudomonas aeruginosa modulates neutrophil granule exocytosis in an in vitro model of airway infection. 文献引用 Immunology and Cell Biology; 100(5) pg352-370 PubMed Id 35318736 第一作者 Liu, W 标题 hUC-MSCs Attenuate Acute Graft-Versus-Host Disease through Chi3l1 Repression of Th17 Differentiation. 文献引用 Stem Cells International; 2022 pg1052166 PubMed Id 36277038 第一作者 Yang, Z 标题 USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade 文献引用 Nature Communications; 12(1) pg4852 PubMed Id 34381028