Biotinylated Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17E. Anti-Human IL-17E specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human IL-17E (PeproTech catalog# 200-24)

Sandwich ELISA: To detect Human IL-17E by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-17E (500-P89Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17E.

Western Blot: To detect Human IL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.  When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17E is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.