Recombinant Human sCD14 0 ComentariosSubmit a Review Detalles del Producto Nùmero de Catalogo 110-01 Descripcion CD14 is a cell surface-anchored glycoprotein that is expressed predominantly by monocytes and tissue macrophages. CD14 associates with MD-2 (LY-96) and TLR4 to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide (LPS) binding. The CD14/MD-2/TLR4 receptor complex signals via MyD88, TIRAP, and TRAF6, and ultimately activates NF-κB. CD14 also exists in a soluble form, designated as sCD14, which is capable of specifically binding LPS in the extracellular space. Recombinant Human sCD14 is a 331 amino acid glycoprotein comprising the extracellular portion of the CD14 receptor. The calculated molecular weight of Recombinant Human sCD14 is 35.6 kDa. Source: HEK293 cells Synonyms: soluble CD14, Monocyte differentiation antigen CD14 AA Sequence: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by the dose-dependent activation of NF-κB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/μl to 200 ng/μl. The NF-κB activation is enhanced when the assay is done in the presence of 0.25 ng/μl to 1.0 ng/μl bacterial LPS. Calculated Molecular Weight: 35.6 kDa Accession Number: P08571 Gene ID: 929 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse, References PubMed SDS Búsqueda del CoA Product Line Country Of Origin: USA Not for human use. Research Interest Immune System Inflammation Receptors product.subtitle.recentcitations Primer autor Saravanan, R Titulo Structural basis for endotoxin neutralisation and anti-inflammatory activity of thrombin-derived C-terminal peptides. Citar Nature Communications; 9(1) pg2762 PudMed id 30018388 Primer autor Andrukhov, O Titulo Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists. Citar Scientific Reports; 7(1) pg12856 PudMed id 28993635 Primer autor Hu, L Titulo Extraordinarily potent proinflammatory properties of lactoferrin-containing immunocomplexes against human monocytes and macrophages. Citar Scientific Reports; 7(1) pg4230 PudMed id 28652573
