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Animal-Free Cytokines

Animal-Free Recombinant Human SCF

제품 상세

카다로그 번호 AF-300-07
제품 설명
Animal-Free Recombinant Human SCF

SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor

AA Sequence: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

Calculated Molecular Weight: 18.4 kDa

Accession Number: P21583

Gene ID: 4254

Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug)

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

제 1 저자
King, A J
주제
Reactivation of a developmentally silenced embryonic globin gene
인용
Nature Communications; 12(1) pg4439
PubMed ID
제 1 저자
Giuli, M V
주제
Notch3 contributes to T-cell leukemia growth via regulation of the unfolded protein response
인용
Oncogenesis; 9(10) pg93
PubMed ID
제 1 저자
Thomé, C H
주제
NTAL is associated with treatment outcome, cell proliferation and differentiation in acute promyelocytic leukemia
인용
Scientific Reports; 10(1) pg10315
PubMed ID

product.subtitle.productreviews

기존 리뷰
For differentiation macrophage progenitors.
I have been using this human SCF for differentiating macrophage progenitors. It worked perfectly, and I definitely recommend.
에서:  Yongjin | 날짜:  2020-08-26