Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEotaxin-3. Anti-Human Eotaxin-3 specific antibody was purified by affinity chromatography employing immobilized hEotaxin-3 matrix.

Immunogen: E.coli derived Recombinant Human Eotaxin-3 (CCL26) (PeproTech catalog# 300-48)

Sandwich ELISA: To detect hEotaxin-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Eotaxin-3 (500-P156GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.

Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.