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Biotinylated Polyclonal Antibodies

Biotinylated Anti-Murine IFN-γ

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Product Details

Catalog Number: 500-P119BT
Description:
Biotinylated Anti-Murine IFN-γ
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Murine IFN-γ. Anti-Murine IFN-γ specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Murine IFN-γ (PeproTech catalog# 315-05)

Sandwich ELISA: To detect Murine IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine IFN-γ (500-P119) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IFN-γ.

Biotinylated Anti-Murine IFN-γ Sandwich ELISA

Western Blot: To detect Murine IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IFN-γ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Murine IFN-γ Western Blot Reduced Biotinylated Anti-Murine IFN-γ Western Blot Unreduced

Note:

1mg will be provided as 2x500μg.

Cross Reactivity Cited in References:
Country Of Origin: USA

Not for human use.

Research Interest

Recent Citations

First Author
Gonzalez-Rey, E
Title
Cortistatin, a new antiinflammatory peptide with therapeutic effect on lethal endotoxemia.
Citation
The Journal of Experimental Medicine; 203(3) pg563-71
PubMed ID
First Author
Lentsch, A B
Title
STAT4 and STAT6 regulate systemic inflammation and protect against lethal endotoxemia.
Citation
The Journal of Clinical Investigation; 108(10) pg1475-82
PubMed ID