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Biotinylated Polyclonal Antibodies

Biotinylated Anti-Murine MIP-2 (CXCL2)

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Product Details

Catalog Number: 500-P130BT
Description:
Biotinylated Anti-Murine MIP-2 (CXCL2)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-2. Anti-Murine MIP-2 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Murine MIP-2 (CXCL2) (PeproTech catalog# 250-15)

Sandwich ELISA: To detect mMIP-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine MIP-2 (500-P130) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mMIP-2.

Biotinylated Anti-Murine MIP-2 (CXCL2) Sandwich ELISA

Western Blot: To detect mMIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Murine MIP-2 (CXCL2) Western Blot Reduced Biotinylated Anti-Murine MIP-2 (CXCL2) Western Blot Unreduced

Note:

1mg will be provided as 2x500μg.

Cross Reactivity Cited in References:
Country Of Origin: USA

Not for human use.

Research Interest

Recent Citations

First Author
Rose, C E
Title
Murine lung eosinophil activation and chemokine production in allergic airway inflammation.
Citation
Cellular & molecular immunology; 7(5) pg361-74
PubMed ID
First Author
Gonzalez-Rey, E
Title
Cortistatin, a new antiinflammatory peptide with therapeutic effect on lethal endotoxemia.
Citation
The Journal of Experimental Medicine; 203(3) pg563-71
PubMed ID
First Author
Lentsch, A B
Title
STAT4 and STAT6 regulate systemic inflammation and protect against lethal endotoxemia.
Citation
The Journal of Clinical Investigation; 108(10) pg1475-82
PubMed ID