Recombinant Human BMP-4 (HeLa derived)

Product Details

Catalog Number: 120-05

Description:

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. Members of this superfamily are widely distributed throughout the body, and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 is involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Recombinant Human BMP-4, expressed in HeLa cells, is a 25.6 kDa homodimeric glycoprotein.

Source: HeLa cells

Synonyms: Bone Morphogenetic Protein-4, BMP-2B, DVR4

AA Sequence (monomer): HHSQRARKKN KNCRRHSLYV DFSDVGWNDW IVAPPGYQAF YCHGDCPFPL ADHLNSTNHA IVQTLVNSVN SSIPKACCVP TELSAISMLY LDEYDKVVLK NYQEMVVEGC GCR

Purity: ≥ 97% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED₅₀ for this effect is 2-8 ng/ml.

Calculated Molecular Weight: 25.6 kDa

Accession Number: P12644

Gene ID: 652

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

Note:

100μg is the largest vial size for this product.
Larger sizes supplied as multiple 50μg and/or 100μg vials.

Cross Reactivity Cited in References:

Human, Mouse, Mouse + Rat, Pig, Rat

Country Of Origin: USA

Not for human use.

Research Interest

Organoids

Angiogenesis/Cardiovascular

Apoptosis

Bone, Skeletal, Cartilage

Cell Culture

Immune System

Neurobiology

Stem Cells & Differentiation

TGF-β Superfamily

Recent Citations

First Author

Kitajima, K

Title

An Interferon-γ/FLT3 Axis Positively Regulates Hematopoietic Progenitor Cell Expansion from Human Pluripotent Stem Cells.

Citation

Stem cells (Dayton, Ohio); 40(10) pg906-918

PubMed ID

35901509

First Author

van der Vaart, J

Title

Modelling of primary ciliary dyskinesia using patient-derived airway organoids.

Citation

EMBO reports; 22(12) pge52058

PubMed ID

34693619

First Author

Pouyanfard, S

Title

Human induced pluripotent stem cell-derived macrophages ameliorate liver fibrosis.

Citation

Stem cells (Dayton, Ohio); 39(12) pg1701-1717

PubMed ID

34460131

Product Reviews

Existing reviews

High-yield iPSC differentiation!

When differentiating stem cells, I always turn to Peprotech for ordering my growth factors (like BMP4). They stay stable for long periods of time under the recommended storage conditions. And they hold up well in the differentiation media, because I get good differentiation results without excessive cell death. Results are consistent between differentiations, so I appreciate this reproducibility. ... Show More

From: Kendall | Date: 26/08/2020

iPSC-Microglia differentiation

When I differentiate iPSC into microglia, I always use peprotech products. It is important to have a good quality of cytokines and growth factors to yield microglia else the iPSC would undergo spontaneous differentiation and non-myeloidal lineages. With BMP-4 from peprotech, I get good consistency in generating mesodermal cells and high yield microglial progenitors cells in large quantities.

From: Raghavendra | Date: 24/08/2021

Growth Factors & Cytokines