Recombinant Murine IL-4

Product Details

Catalog Number: 214-14

Description:

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant Murine IL-4 is a 13.5 kDa globular protein containing 121 amino acid residues.

Source: E.coli

Synonyms: BCGF, BCDF, B cell stimulating factor (BSF-1)

AA Sequence: MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED₅₀ was determined by the dose-dependent proliferation of murine HT-2 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁵ units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 13.5 kDa

Accession Number: P07750

Gene ID: 16189

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

Cross Reactivity Cited in References:

Bacteria, Human, Human + Mouse, Leech, Mouse, Mouse + Virus, Parasite, Rat, Trout

Country Of Origin: USA

Not for human use.

Research Interest

Stem Cells & Differentiation

Allergy

Transplantation

Recent Citations

First Author

Tu, Q

Title

Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling.

Citation

Cell Proliferation; 55(12) pge13328

PubMed ID

36106559

First Author

Li, Y

Title

Targeted inhibition of STAT3 induces immunogenic cell death of hepatocellular carcinoma cells via glycolysis.

Citation

Molecular Oncology; 16(15) pg2861-2880

PubMed ID

35665592

First Author

Hanuscheck, N

Title

Interleukin-4 receptor signaling modulates neuronal network activity.

Citation

The Journal of Experimental Medicine; 219(6)

PubMed ID

35587822

Product Reviews

Existing reviews

works well

Used to polarize RAW 264.7 macrophages.

From: Akil | Date: 08/07/2020

Good

Fast shipping, good quantity.

From: Sandra | Date: 08/07/2020

Very Good

Used to M2 macrophage polarization and always works well.

From: Yingjun | Date: 21/07/2020

Product with great consistency

We have been using this cytokine for immune cells extract from mouse spleen and bone marrow in numerous publications for the past 10 years. Great consistency!

From: Jin | Date: 21/07/2020

Great consistent IL-4 cytokine for differentiation

We routinely use this cytokine to generate mouse CD4+ Th2 cells and for M2 macrophage polarization. The pricing and consistency of this cytokine is why we have stuck with peprotech for almost 20 years. Peprotech is our sole vendor for all cytokines.

From: Chirag | Date: 31/08/2020

Growth Factors & Cytokines