Recombinant Murine LIF 0 ReviewsSubmit a Review Product Details Catalog Number: 250-02 Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds. Source: E.coli Synonyms: Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI), Interleukin 6 family cytokine AA Sequence: SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: PeproTech's Murine LIF is fully biologically active when compared to standards. The ED50 as determined by the M1 cell differentiation assay is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. Calculated Molecular Weight: 19.6 kDa Accession Number: P09056 Gene ID: 16878 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Diabetes/Weight Regulation Immune System Lipid Metabolism Stem Cells & Differentiation Recent Citations First Author Zhu, Y Title Cell cycle heterogeneity directs spontaneous 2C state entry and exit in mouse embryonic stem cells. Citation Stem Cell Reports; 16(11) pg2659-2673 PubMed ID 34624246 First Author Wulansari, N Title LIF maintains mouse embryonic stem cells pluripotency by modulating TET1 and JMJD2 activity in a JAK2-dependent manner. Citation Stem cells (Dayton, Ohio); 39(6) pg750-760 PubMed ID 33529470 First Author Panda, S Title IL-4 controls activated neutrophil FcγR2b expression and migration into inflamed joints. Citation Proceedings of the National Academy of Sciences of the United States of America; 117(6) pg3103-3113 PubMed ID 31980518
