Animal-Free Recombinant Murine TNF-α 0 ReviewsSubmit a Review Product Details Catalog Number: AF-315-01A Description: TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Murine TNF-α is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin AA Sequence: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. Calculated Molecular Weight: 17.3 kDa Accession Number: P06804 Gene ID: 21926 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Angiogenesis/Cardiovascular Apoptosis Cancer Diabetes/Weight Regulation Immune System Inflammation Neurobiology Stem Cells & Differentiation TNF Superfamily Allergy Transplantation Recent Citations First Author Sonner, J Title Dietary tryptophan links encephalogenicity of autoreactive T cells with gut microbial ecology. Citation Nature Communications; 10(1) pg4877 PubMed ID 31653831 First Author Mandal, P Title Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock. Citation Immunity; 49(1) pg42-55.e6 PubMed ID 30021146 First Author Pepin, M Title Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. Citation Scientific Reports; 6 pg37953 PubMed ID 27892504
