Anti-Human Galectin-1 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P210 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGalectin-1. Anti-Human Galectin-1 specific antibody was purified by affinity chromatography employing immobilized hGalectin-1 matrix. Immunogen: E.coli derived Recombinant Human Galectin-1 (PeproTech catalog# 450-39) Sandwich ELISA: To detect hGalectin-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Galectin-1 (500-P210Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1. Western Blot: To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Chicken, Hamster, Human, Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Cancer Immune System product.subtitle.recentcitations First Author Sanjurjo, L Title Chemokines modulate glycan binding and the immunoregulatory activity of galectins. Citation Communications Biology; 4(1) pg1415 PubMed Id 34931005 First Author Posada, I M Title Opposite feedback from mTORC1 to H-ras and K-ras4B downstream of SREBP1. Citation Scientific Reports; 7(1) pg8944 PubMed Id 28827765 First Author BlaževitÅ¡, O Title Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering. Citation Scientific Reports; 6 pg24165 PubMed Id 27087647