Antigen-affinitätsgereinigte Polyklonale Antikörper

Anti-Human IL-1β

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Product Details

Catalogue Number: 500-P21BG
Description:
Anti-Human IL-1β
 

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-1β. Anti-Human IL-1β specific antibody was purified by affinity chromatography employing immobilized hIL-1β matrix.

Immunogen: E.coli derived Recombinant Human IL-1β (PeproTech catalog# 200-01B)

Sandwich ELISA: To detect hIL-1β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-1β (500-P21BGBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-1β.

Anti-Human IL-1β Sandwich ELISA

Western Blot: To detect hIL-1β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-1β Western Blot Reduced Anti-Human IL-1β Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Blanco-Melo, D
Title
Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.
Citation
Cell; 181(5) pg1036-1045.e9
PubMed Id
First Author
Chong, H C
Title
Regulation of epithelial-mesenchymal IL-1 signaling by PPARbeta/delta is essential for skin homeostasis and wound healing.
Citation
The Journal of Cell Biology; 184(6) pg817-31
PubMed Id
First Author
Ferrari, C C
Title
Reversible demyelination, blood-brain barrier breakdown, and pronounced neutrophil recruitment induced by chronic IL-1 expression in the brain.
Citation
The American Journal of Pathology; 165(5) pg1827-37
PubMed Id