Produced in BALB/c x C57BL/6 F1 mice immunized with highly pure Recombinant Human IL-15. Anti-Human IL-15-specific antibody was purified from ammonium sulfate precipitation followed by ion exchange chromatography.
E.coli derived Recombinant Human IL-15 (PeproTech catalog# 200-15)
In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 250pg/ml Recombinant Human IL-15 when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human IL-15 (500-P15BT) as the detection antibody at a concentration of at least 2.0-4.0 μg/ml.
To detect Human IL-15 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml (non-reducing) or at a concentration of 2.0-4.0 μg/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-15 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.