Antigen-affinitätsgereinigte Polyklonale Antikörper

Anti-Human MIP-4 (CCL18)

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Product Details

Catalogue Number: 500-P108

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-4. Anti-Human MIP-4 specific antibody was purified by affinity chromatography employing immobilized hMIP-4 matrix.

Immunogen: E.coli derived Recombinant Human MIP-4 (CCL18) (PeproTech catalog# 300-34)

Sandwich ELISA: To detect hMIP-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MIP-4 (500-P108Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4.

Anti-Human MIP-4 (CCL18) Sandwich ELISA

Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human MIP-4 (CCL18) Western Blot Reduced Anti-Human MIP-4 (CCL18) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Schutyser, E
Title
Identification of biologically active chemokine isoforms from ascitic fluid and elevated levels of CCL18/pulmonary and activation-regulated chemokine in ovarian carcinoma.
Citation
The Journal of Biological Chemistry; 277(27) pg24584-93
PubMed Id
First Author
Vulcano, M
Title
Unique regulation of CCL18 production by maturing dendritic cells.
Citation
Journal of immunology (Baltimore, Md. : 1950); 170(7) pg3843-9
PubMed Id
First Author
Struyf, S
Title
PARC/CCL18 is a plasma CC chemokine with increased levels in childhood acute lymphoblastic leukemia.
Citation
The American Journal of Pathology; 163(5) pg2065-75
PubMed Id