Produced from sera of rabbits immunized with highly pure Recombinant Human MMP-2. Anti-Human MMP-2-specific antibody was purified by affinity chromatography employing an immobilized Human MMP-2 matrix.
E.coli-derived, 62.0 kDa Recombinant Human MMP-2 (PeproTech catalog# 420-02)
To detect Human MMP-2 by sandwich ELISA (using 100ul/well) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MMP-2 (500-P307BT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human MMP-2.
To detect Human MMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MMP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.
Not for human use.