Produced from sera of rabbits immunized with highly pure Recombinant Human Resistin. Anti-Human Resistin specific antibody was purified by affinity chromatography employing immobilized Human Resistin matrix.
E.coli derived Recombinant Human Resistin (PeproTech catalog# 450-19)
To detect Human Resistin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Resistin (500-P183Bt) as a detection antibody, allows the detection of at least 2000-4000 ng/well of Recombinant Human Resistin.
To detect Human Resistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Resistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.