Anti-Human VEGF165 (Polyclonal Rabbit) 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P10 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Human VEGF 165 matrix. Immunogen: E.coli derived Recombinant Human VEGF165 (PeproTech catalog# 100-20) Sandwich ELISA: To detect hVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human VEGF (500-P10Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Hamster, Human, Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Cancer Inflammation Proliferation product.subtitle.recentcitations First Author Hamilton, B J Title Phorbol esters induce PLVAP expression via VEGF and additional secreted molecules in MEK1-dependent and p38, JNK and PI3K/Akt-independent manner. Citation Journal of Cellular and Molecular Medicine; 23(2) pg920-933 PubMed Id 30394679 First Author Meda, C Title Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages. Citation Journal of immunology (Baltimore, Md. : 1950); 188(8) pg4081-92 PubMed Id 22442441 First Author Bottos, A Title Targeting oncogenic serine/threonine-protein kinase BRAF in cancer cells inhibits angiogenesis and abrogates hypoxia. Citation Proceedings of the National Academy of Sciences of the United States of America; 109(6) pgE353-9 PubMed Id 22203991
