Antigen-affinitätsgereinigte Polyklonale Antikörper

Anti-Murine JE/MCP-1 (CCL2)

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Product Details

Catalogue Number: 500-P113

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mJE(MCP-1). Anti-Murine JE(MCP-1) specific antibody was purified by affinity chromatography employing immobilized mJE (MCP-1) matrix.

Immunogen: E.coli derived Recombinant Murine JE/MCP-1 (CCL2) (PeproTech catalog# 250-10)

Sandwich ELISA: To detect mJE(MCP-1) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine JE(MCP-1)(500-P113Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).

Anti-Murine JE/MCP-1 (CCL2) Sandwich ELISA

Western Blot: To detect mJE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE(MCP-1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine JE/MCP-1 (CCL2) Western Blot Reduced Anti-Murine JE/MCP-1 (CCL2) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Roodveldt, C
Title
Glial innate immunity generated by non-aggregated alpha-synuclein in mouse: differences between wild-type and Parkinson's disease-linked mutants.
Citation
PLoS ONE; 5(10) pge13481
PubMed Id
First Author
Stowe, A M
Title
CCL2 upregulation triggers hypoxic preconditioning-induced protection from stroke.
Citation
Journal of Neuroinflammation; 9 pg33
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First Author
Lobo, P I
Title
Natural IgM anti-leukocyte autoantibodies attenuate excess inflammation mediated by innate and adaptive immune mechanisms involving Th-17.
Citation
Journal of immunology (Baltimore, Md. : 1950); 188(4) pg1675-85
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