Antigen-affinitätsgereinigte Polyklonale Antikörper

Anti-Rat IL-1β

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Product Details

Catalogue Number: 500-P80

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Rat IL‑1β.  Anti-Rat IL‑1β specific antibody was purified by affinity chromatography employing an immobilized Rat IL‑1β matrix.

Immunogen: E.coli derived Recombinant Rat IL-1β (PeproTech catalog# 400-01B)

Sandwich ELISA: To detect Rat IL-1β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat IL-1β (500-P80Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1β.

Anti-Rat IL-1β Sandwich ELISA

Western Blot: To detect Rat IL-1β by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat IL-1β Western Blot Reduced Anti-Rat IL-1β Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Rat
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Chen, Z
Title
CCR5 signaling promotes lipopolysaccharide-induced macrophage recruitment and alveolar developmental arrest
Citation
Cell Death & Disease; 12(2) pg184
PubMed Id
First Author
Foright, R M
Title
Compensatory eating behaviors in male and female rats in response to exercise training.
Citation
American journal of physiology. Regulatory, integrative and comparative physiology; 319(2) pgR171-R183
PubMed Id