Biotinylated Anti-Human IL-4 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P24BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-4. Anti-Human IL-4 specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Human IL-4 (PeproTech catalog# 200-04) Sandwich ELISA: To detect hIL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IL-4 (500-P24) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-4. Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Apoptosis Cancer Cell Culture Immune System Inflammation Proliferation Stem Cells & Differentiation Allergy Transplantation product.subtitle.recentcitations First Author Blanco-Melo, D Title Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19. Citation Cell; 181(5) pg1036-1045.e9 PubMed Id 32416070
