Produced from sera of rabbits immunized with highly pure Recombinant Human PD-L1 Fc. Anti-Human PD-L1 Fc-specific antibody was purified by affinity chromatography and then biotinylated.
CHO cells derived Recombinant Human PD-L1 Fc (PeproTech catalog# 310-35)
To detect Human PD-L1 Fc by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human PD-L1 Fc (500-P321) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PD-L1 Fc.
To detect Human PD-L1 Fc by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human PD-L1 Fc is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.