Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human CD14-specific antibody was purified by affinity chromatography and then biotinylated.
HEK293 cells derived Recombinant Human sCD14 (PeproTech catalog# 110-01)
To detect Human sCD14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human sCD14 (500-P320) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human sCD14.
To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sCD14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.