Biotinylierte Polyklonale Antikörper

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Human SDF-1β. Anti-Human SDF-1β specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli-derived, 7.9 kDa Recombinant Human SDF-1β (CXCL12) (PeproTech catalog# 300-28B)

Sandwich ELISA: To detect Human SDF-1β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human SDF-1β (500-P87BG) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human SDF-1β.

Western Blot: To detect Human SDF-1β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.