Biotinylated Anti-Murine IL-10 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P60BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-10. Anti-Murine IL-10 specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Murine IL-10 (PeproTech catalog# 210-10) Sandwich ELISA: To detect mIL-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine IL-10 (500-P60) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-10. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Cancer Immune System Inflammation Neurobiology Stem Cells & Differentiation Allergy Transplantation product.subtitle.recentcitations First Author Verginis, P Title Delineation of five thyroglobulin T cell epitopes with pathogenic potential in experimental autoimmune thyroiditis. Citation Journal of immunology (Baltimore, Md. : 1950); 169(9) pg5332-7 PubMed Id 12391254
