Registration for PeproTech.com is no longer available. To order your PeproTech products online, register or logon to ThermoFisher.com.

Biotinylierte Polyklonale Antikörper

Biotinylated Anti-Rat TNF-α

0 BewertungenSubmit a Review

Produktdetails

Katalognummer: 500-P72BT
Beschreibung:
Biotinylated Anti-Rat TNF-α
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Rat TNF-α. Anti-Rat TNF-α specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli-derived, 17.3 kDa Recombinant Rat TNF-α (PeproTech catalog# 400-14)

Sandwich ELISA: To detect Rat TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat TNF-α (500-P72) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat TNF-α.

Biotinylated Anti-Rat TNF-alpha Sandwich ELISA

Western Blot: To detect Rat TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Rat TNF-α Western Blot Reduced Biotinylated Anti-Rat TNF-α Western Blot Unreduced

Note:

1mg will be provided as 2x500μg.

Country Of Origin: USA

Not for human use.

Research Interest