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Biotinylierte Polyklonale Antikörper

Biotinylated Anti-Rat VEGF165

Produktdetails

Katalognummer: 500-P275BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat VEGF (Rat Vascular Endothelial Growth Factor). Anti-Rat VEGF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli-derived, 38.5 kDa Recombinant Rat VEGF165 (PeproTech catalog# 400-31)

Sandwich ELISA: To detect Rat VEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat VEGF (500-P275) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat VEGF.

Western Blot: To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.

Country Of Origin: USA

Not for human use.

Research Interest