PeproGrow™ hESC (Embryonic Stem Cell) Basal Medium

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Product Details

Catalogue Number: BM-HESC

PeproTech’s PeproGrow™ hESC Media products are comprised of complete, chemically-defined formulations designed for the feeder-free expansion and maintenance of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). Intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (Nanog+/Lin28+/Tra-1-60+/SSEA1-/SSEA4+/Oct4+), these formulations demonstrate less than 15% spontaneous differentiation as indicated by immunofluorescent staining and flow cytometry. These proprietary formulations were designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University. Each PeproGrow™ hESC Medium Kit includes a bottle of basal medium and a separate, lyophilized component of PeproTech’s recombinant growth factors. Additional companion products are available separately and include the Animal-Free Human Vitronectin Matrix and Buffer Kit (catalog number AF-VMB-220), and the Cell Passaging/Non-Enzymatic Detachment Buffer (catalog number CPD-125). PeproTech’s Animal-Free Human Vitronectin Matrix and Buffer Kit can be used following either a surface-coating or premix method.

PeproGrow™ hESC Medium Kit is an insulin-free formulation, in which insulin is replaced by a unique activator of similar pathways to eliminate possible interference during the measurement of insulin-production in subsequently differentiated cells.

This product listing is for a bottle of the basal medium. The lyophilized growth factor component can be found here.

Note: On December 1, 2022 this product will be discontinued. Gibco™ Essential 8™ Medium (Catalog# A1517001) can be used as a substitution for this product.

Human Embryonic Stem Cell Basal Medium

Note: The manual for this basal medium can be found here

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Hanna, R
G-quadruplexes originating from evolutionary conserved L1 elements interfere with neuronal gene expression in Alzheimer's disease
Nature Communications; 12(1) pg1828
PubMed Id