Animal-Free Recombinant Human G-CSF

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Katalognummer: AF-300-23

Beschreibung:

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant Human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Granulocyte Colony-Stimulating Factor, CSF-3, MGI-1G, GM-CSFβ, pluripoietin

AA Sequence: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to stimulate the proliferation of mouse NFS-60 cells. The expected ED₅₀ is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 18.7 kDa

Accession Number: P09919

Gene ID: 1440

Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug)

crossreactivity:

Human, Mouse, Plant