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Recombinant Human IFN-γ

Product Details

Catalogue Number: 300-02
Description:

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells.  IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins.  This promotes the presentation of antigen to T-helper (CD4+) cells.  IFN-γ signaling in antigen-presenting cells, and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response.  Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils.  Human IFN-γ is species-specific and is biologically active only in human and primate cells.  Recombinant Human IFN-γ is a 16.8 kDa protein containing 144 amino acid residues.

Source: E.coli

Synonyms: Immune Interferon, type II interferon, T cell interferon, MAF

AA Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Assay #1: Determined by its ability to induce STAT1/STAT2 activation in Human COLO 205 ISRE LUC reporter cells.

Assay #2: The ED50 determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 16.8 kDa

Accession Number: P01579.1

Gene ID: 3458

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Bodaghi, B
Title
Role of IFN-gamma-induced indoleamine 2,3 dioxygenase and inducible nitric oxide synthase in the replication of human cytomegalovirus in retinal pigment epithelial cells.
Citation
Journal of immunology (Baltimore, Md. : 1950); 162(2) pg957-64
PubMed Id
First Author
Gupta, S
Title
Signaling pathways mediated by the TNF- and cytokine-receptor families target a common cis-element of the IFN regulatory factor 1 promoter.
Citation
Journal of immunology (Baltimore, Md. : 1950); 161(11) pg5997-6004
PubMed Id
First Author
Suda, T
Title
Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing.
Citation
The Journal of Experimental Medicine; 186(12) pg2045-50
PubMed Id

product.subtitle.productreviews

Existing reviews
Confirmed dose response
Used in an experiment and saw a nice dose response effect
From:  Lucia | Date:  24.08.2021