IL-23 is a proinflammatory, heterodimeric protein composed of two subunits: a unique p19 subunit, and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated dendritic cells and macrophages, and signals though a receptor comprised of IL-23R complexed with IL-12Rβ1. IL-23 has been shown to enhance proliferation of memory T cells. It also stimulates the production of IFN-γ in NK cells, induces IL-17 production, and drives Th17-mediated responses. Recombinant Human IL-23 is a 53.5 kDa, heterodimeric protein consisting of the two subunits, p19 (170 amino acids) and p40 (306 amino acids).
(BTI-Tn-5B1-4) Hi-5 Insect cells
p19 Subunit: RAVPGGSSPA WTQCQQLSQK LCTLAWSAHP LVGHMDLREE GDEETTNDVP HIQCGDGCDP QGLRDNSQFC LQRIHQGLIF YEKLLGSDIF TGEPSLLPDS PVGQLHASLL GLSQLLQPEG HHWETQQIPS LSPSQPWQRL LLRFKILRSL QAFVAVAARV FAHGAATLSP
p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
≥ 95% by SDS-PAGE gel and HPLC analyses.
Determined by its ability to stimulate the proliferation of HEK-Blue™ IL-23 reporter cells.
Calculated Molecular Weight:
p19: Q9NPF7 p40: P29460
p19: 51561 p40: 3593
Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)
1mg will be provided as 2x500μg.
Not for human use.