Chemokine

Recombinant Human SDF-1α (CXCL12)

Product Details

Catalogue Number: 300-28A
Description:

SDF-1α and β are stromal-derived, CXC chemokines that signal through the CXCR4 receptor. SDF-1α and β chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV-suppressive activity in cells expressing the CXCR4 receptor. Human and murine SDF-1 proteins act across species. SDF-1α and β contain the four highly conserved cysteine residues present in CXC chemokines. The mature SDF-1α protein is the result of alternative splicing of the SDF-1 gene and contains 68 amino acid residues. Recombinant Human SDF-1α is an 8.0 kDa protein containing 68 amino acid residues.

Source: E.coli

Synonyms: Stromal-Cell Derived Factor-1, CXCL12, PBSF

AA Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to chemoattract IL-2-activated human T cells using a concentration range of 20.0-80.0 ng/ml.

Calculated Molecular Weight: 8 kDa

Accession Number: P48061

Gene ID: 6387

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Stanley, P
Title
Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.
Citation
The EMBO Journal; 27(1) pg62-75
PubMed Id
First Author
Alekhina, O
Title
β-Arrestin1 and Signal-transducing Adaptor Molecule 1 (STAM1) Cooperate to Promote Focal Adhesion Kinase Autophosphorylation and Chemotaxis via the Chemokine Receptor CXCR4.
Citation
The Journal of Biological Chemistry; 291(50) pg26083-26097
PubMed Id
First Author
Mandawat, A
Title
Pan-histone deacetylase inhibitor panobinostat depletes CXCR4 levels and signaling and exerts synergistic antimyeloid activity in combination with CXCR4 antagonists.
Citation
Blood; 116(24) pg5306-15
PubMed Id

product.subtitle.productreviews

Existing reviews
Migration assay
We have been using this chemokine to test the ability of our cells to migrate and we always get really good results when working with PBMCs. Highly recommended.
From:  Jhomary | Date:  26.08.2020