Animal-Free Recombinant Human Vitronectin 0 BewertungenSubmit a Review Produktdetails Katalognummer: AF-140-09 Beschreibung: Vitronectin is a secreted glycoprotein that is synthesized in the liver. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide-linked multimers. The multimeric vitronectin can efficiently bind to, and incorporate into, the extracellular matrix. Within the matrix, vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Additionally, recombinant vitronectin can function as a chemically-defined matrix component in human embryonic stem cell renewal media. Recombinant Human Vitronectin is a 459 amino acid, single-chain, monomeric protein, which migrates at an apparent molecular weight of 75 kDa by SDS-PAGE under reducing conditions. The calculated molecular weight of Recombinant Human Vitronectin is 52.2 kDa. Manufactured using all Animal-Free reagents. Source: HEK293 cells Synonyms: VTN, Serum-spreading factor, V75 AA Sequence: DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Calculated Molecular Weight: 52.2 kDa Biological Activity: Recombinant human Vitronectin promotes attachment of hESC and iPSC in serum-free, feeder conditions at 5ug/ml. Accession Number: P04004 Gene ID: 7448 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Human, References PubMed SDS Datenblattsuche Product Line Country Of Origin: USA Not for human use. Research Interest Immune System Neurobiology Stem Cells & Differentiation product.subtitle.recentcitations Erstautor Paulus, J Titel RGD Peptidomimetic MMAE-Conjugate Addressing Integrin αVβ3-Expressing Cells with High Targeting Index. Literaturstelle Chemistry (Weinheim An Der Bergstrasse, Germany); 29(12) pge202203476 PubMed ID 36454662 Erstautor Lavoie, N S Titel Human induced pluripotent stem cells integrate, create synapses and extend long axons after spinal cord injury. Literaturstelle Journal of Cellular and Molecular Medicine; 26(7) pg1932-1942 PubMed ID 35257489 Erstautor Walsh, P Titel Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population. Literaturstelle Stem cells (Dayton, Ohio); PubMed ID 32745311
