Recombinant Human/Murine/Rat BDNF

Product Details

Catalogue Number: 450-02

Description:

BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues. Recombinant Human/Murine/Rat BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions.

Source: E.coli

Synonyms: Brain Derived Neurotrophic Factor, Abrineurin

AA Sequence (monomer): MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 27 kDa

Accession Number: P23560

Gene ID: 627

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

crossreactivity:

Chicken, Frog, Guinea Pig, Hamster, Human, Human + Mouse, Human + Rat, Human + Virus, Monkey, Mouse, Rat, Tadpole, tests

Country Of Origin: USA

Not for human use.

Research Interest

Organoids

Neurobiology

Stem Cells & Differentiation

product.subtitle.recentcitations

First Author

Mesci, P

Title

SARS-CoV-2 infects human brain organoids causing cell death and loss of synapses that can be rescued by treatment with Sofosbuvir.

Citation

PLoS Biology; 20(11) pge3001845

PubMed Id

36327326

First Author

Lee, Y

Title

Mitochondrial genome mutations and neuronal dysfunction of induced pluripotent stem cells derived from patients with Alzheimer's disease.

Citation

Cell Proliferation; 55(7) pge13274

PubMed Id

35698260

First Author

Sabate-Soler, S

Title

Microglia integration into human midbrain organoids leads to increased neuronal maturation and functionality.

Citation

Glia; 70(7) pg1267-1288

PubMed Id

35262217

product.subtitle.productreviews

Existing reviews

I have used PeproTech BDNF since 2012

I have used PeproTech BDNF at Stanford and Genentech since 2012 and it has been great for differentiating stem cells into neurons. It is the best product in this category of recombinant proteins.

From: Louise | Date: 21.08.2020

Supports Growth of Cholinergic Neurons

Routinely use this to support and maintain primary murine cholinergic neurons in culture. Excellent product.

From: Bernadeta | Date: 26.08.2020

Differentiation and support of SH-SY5Y cells

I have been using this product for over 3 years now to support and differentiate SH-SY5Y cells in culture. It is an excellent product that many people in my lab use. It always works well and is reliable.

From: Crystal | Date: 29.08.2020

Works great!

Used this product for intravitreal injections in the mouse eye and it worked as anticipated. Very pleased and will continue to purchase for future experiments.

From: Rebecca | Date: 03.08.2021

Supports Growth of Motor Neurons

I use this product for supporting the maturation of MNs in vitro, it works well.

From: meimei | Date: 04.08.2021

Cortical Neuron Differentiation

Very consistent for stem cell culturing

From: Danielle | Date: 24.08.2021

Great recombinant protein

This is a very good recombinant protein to elicit TrkB signaling in cultured neurons at 50 ng/ml concentration or support differentiation of mouse ESC-s to neurons. We haven’t experienced lot to lot variation and delivery is always prompt. We highly recommend.

From: Epp | Date: 31.08.2021

Neurotrophine